Human Anti-Human VEGF Recombinant Antibody; Fab Fragment (TP-104CL)
CAT#: TP-104CL
This product is a recombinant humanized IgG1 kappa isotype monoclonal antibody fragment designed for intraocular use. The antibody binds to and inhibits the biologic activity of human vascular endothelial growth factor A (VEGF-A). It has a molecular weight of approximately 48 kilodaltons and is produced by an E. coli expression system in a nutrient medium containing the antibiotic tetracycline (tetracycline is not detectable in the final product).
Tested Data
WB
Figure 1 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in WB
Western blot analysis of TP-104CL was performed with Recombinant Human VEGF protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-104CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in ELISA
ELISA analysis of TP-104CL was performed by coating with Recombinant Human VEGF Protein. Then blocked with BSA, and incubated with Anti-VEGF Therapeutic Antibody (ranibizumab) (TP-104CL). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 3 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in DB
Antigen: Human VEGF protein
Antibody incubation concentration: 2ng/μL.
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Specifications
- Type
- Biotech
Target
- Function
- Humanized mAb fragment that binds isoforms of vascular endothelial growth factor A (VEGFA)
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Excellent for VEGF Studies
This Fab fragment has dramatically improved our experiments involving VEGF detection. Its high specificity and consistent performance in ELISA assays allow us to quantify VEGF levels precisely, providing invaluable data for our angiogenesis-related research projects.
Essential for Angiogenesis Research
Utilizing this antibody, we're able to dissect the molecular underpinnings of angiogenesis comprehensively. The Fab fragment's exacting binding to VEGF-A ensures we gain accurate insights into its role within tumor growth models, aiding in the identification of novel anti-angiogenic strategies.
Valuable Research Asset
Our studies of VEGF-mediated pathways have greatly benefited from this Fab fragment. It consistently facilitates accurate and reproducible results in our ELISA and Dot Blot assays, making it an indispensable asset for any project focused on vascular biology.
Q&As
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Which research applications is this Fab fragment most suited for?
A: The Fab fragment is optimized for use in Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting, and Dot Blot assays. It provides precise detection of VEGF-A in these experimental settings, facilitating advancements in understanding angiogenic signaling.
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Why is VEGF important in cancer research?
A: VEGF is a key molecule promoting blood vessel growth. In cancer research, understanding VEGF interactions helps delineate how tumors enhance their blood supply to sustain growth, offering insights into potential therapeutic targets to inhibit tumor angiogenesis.
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What advantages does the recombinant Fab fragment offer over full-length antibodies?
A: The Fab fragment offers reduced size for better tissue penetration in some assays and provides high specificity with minimal Fc-mediated effects. This makes it suitable for applications where steric hindrance or Fc-related interactions may be a concern.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TP-104CL, RRID: AB_3112056)
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Biosimilar Overview
Please refer to Ranibizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ranibizumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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